Description
Principle of the Assay: The PCR Clean-Up & Gel Extraction Kit provides a cost-effective system for the fast and easy isolation of the DNA fragments from PCR reactions, agarose gels, or enzymatic reactions. The DNA fragments (100bp~10Kb) in the special buffers are bound by the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and the pure DNA is eluted with the Tris buffer or water without phenol extraction or alcohol precipitation. The DNA purified with the kits is suitable for any subsequent application, such as ligation and transformation, sequencing, restriction enzyme digestion, labeling, PCR, in vitro transcription, or microinjection. The entire procedure can be completed within 15~20 minutes